000 04049ntm a2200373 i 4500
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005 20251117113400.0
008 190222t20182018my da f am 000 0 eng d
020 _aTHE0000172(Local)
039 9 _a201905131623
_bnazirah
_y201902221557
_zsaini
040 _aUMP
_beng
_cUMP
_erda
090 _aFIST .M87 2018 r Thesis
100 1 _aMurugan, Amarchand Chordia,
_eauthor.
245 1 0 _aStudy on antioxidant and antidiabetic properties of phloroglucinol from brown macroalgae Padina australis Hauck /
_cAmarchand Chordia Murugan
246 3 _aStudy of antioxidant and antidiabetic properties of phloroglucinol from Padina australis Hauck
264 1 _aKuantan, Pahang :
_bUMP,
_c2018
264 4 _c© 2018
300 _axx, 116 pages :
_billustrations (some color), chart ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Industrial Sciences and Technology
502 _aThesis (Doctor of Philosophy in Biotechnology) -- Universiti Malaysia Pahang – 2018
504 _aIncludes bibliographical references
520 3 _aDiabetes is a metabolic disorder characterized by high levels of blood glucose. It is caused by the pancreatic insufficiency or by insulin resistance. Marine macroalgae extracts have been established to have strong antidiabetic and antioxidant properties. In the present study, the antidiabetic and antioxidant potential of polyphenolic extract from a brown seaweed Padina australis was evaluated. Methanolic (80:20 % of MeOH: Deionized Water v/v) extract of P. australis was partitioned with five different solvents. The carbolytic enzyme inhibiting functions, radical scavenging activity assay against 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), ferrous ion chelating (FIC) activity, beta-carotene bleaching properties are evaluated. The antiradical and antidiabetic inhibition assays were statistically significant as the different extracts of P. australis inhibited the enzymes in vitro and in vivo. Among the five different fractions, n-butanol fraction showed significantly higher antiradical and antidiabetic inhibitory activity. The methanolic extract was subjected to TLC and HPLC analysis to identify the bioactive molecules present in the mixture of extract. Further LC-MS analysis of n-butanol 5th fraction revealed the presence of three Phlorotannin compounds namely Phloroglucinol, Eckol, and Phlorofucofuroeckol A. Sephadex LH-20, column chromatography technique, was employed to isolate Phloroglucinol compound for further study. Phloroglucinol isolated and purified from the active fraction was evaluated for antidiabetic effect in Streptozotocin-induced diabetic rats. Among the tested portions, n-butanol fraction had significant α- glucosidase and α-amylase inhibitory activity with IC50 value 2.06 ± 0.14 and 2.90 ± 0.08 mg/mL respectively, as compared to Acarbose. Acute toxicity of the isolated pure compound at 20 and 40 mg/kg doses in Swiss Albino mice was found to have potent antidiabetic activity by ameliorating glucose, lipids, and antioxidant parameters. The toxicology studies also showed that the pure compound Phloroglucinol did not have any toxic effects in the internal organs; biochemical and hematological parameters. These findings suggest that the dietary use of Padina australis and the isolated compound Phloroglucinol can be used as medicine for the treatment of diabetes. This work could help to develop new oral antihyperglycemic agent in the management of diabetes mellitus.
610 2 0 _aFaculty of Industrial Sciences and Technology
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c7726
_d7732
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2640*2641*3000*3360*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992