000 03029ntm a2200349 i 4500
001 vtls000103684
003 KUKTEM
005 20251117113402.0
008 180531s2018 my a f am 000 0 eng d
020 _aTHE0001430(Local)
039 9 _a201905211441
_basmadi
_y201805311438
_zsaini
040 _aUMP
_beng
_cUMP
_erda
090 _aFTeK .T363 2018 r Bc.
100 1 _aTan, Darlene Tzet Lene,
_eauthor.
245 1 0 _aExtraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry /
_cDarlene Tan Tzet Lene
264 1 _aKuantan, Pahang :
_bUMP,
_c2018
300 _axiii, 71 pages :
_billustrations (some color) ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Engineering Technology
502 _aProject Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2018
504 _aIncludes bibliographical references
520 3 _aThe objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at 𝑅𝑓 value of 0.22. However, in HPLC analysis, the caffeic acid was not detected in sample at the retention time of 3.82 minutes as per reference of the standard caffeic acid chromatogram. Split peak and baseline drift were also observed in WEP during HPLC analysis and requires troubleshooting process. WEP showed lower amount of total flavone, flavonols and total phenolic contents as compared to ethanolic extracted peopolis (EEP) and olive oil extracted propolis (OEP). For TLC, chrysin components was detected in EEP whereas none of the components were detected by OEP. For HPLC, caffeic acid were not detected all three propolis extracts.
610 2 0 _aFaculty of Engineering Technology
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c7764
_d7770
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2640*3000*3360*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992