000 02867ntm a2200349 i 4500
001 vtls000103753
003 KUKTEM
005 20251117113404.0
008 180604s2017 my a f am 000 0 eng d
020 _aTHE0001437(Local)
039 9 _a201905211450
_basmadi
_c201806041114
_dsaini
_y201806041104
_zsaini
040 _aUMP
_beng
_cUMP
_erda
090 _aFTeK .W66 2017 r Bc.
100 1 _aWong, Shin Yie,
_eauthor.
245 1 0 _aStatistical analysis of factors affecting monoclonal antibody production by using principal component analysis :
_bphysiological characteristics of cell line /
_cWong Shin Yie
264 1 _aKuantan, Pahang :
_bUMP,
_c2017
300 _axiii, 71 pages :
_billustrations (some color) ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Engineering Technology
502 _aProject Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2017
504 _aIncludes bibliographical references
520 3 _aRapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential.
610 2 0 _aFaculty of Engineering Technology
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c7819
_d7825
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2640*3000*3360*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992