000 03356ntm a2200361 i 4500
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005 20251117113405.0
008 180926t20182018my a f a m 001 0 eng d
020 _aTHE0000823(Local)
039 9 _a201905271210
_bnazirah
_y201809261140
_zfateeha
040 _aUMP
_beng
_cUMP
_erda
090 _aFKKSA .A35 2018 r Thesis
100 0 _aNoor Adilah Md Salehan,
_eauthor.
245 1 0 _aAn integrated ultrasonic-assisted enzymes as a single extraction unit for gallic acid from labisia pumila (kacip fatimah) /
_cNoor Adilah Md Salehan
264 1 _aKuantan, Pahang :
_bUMP,
_c2018
264 4 _c© 2018
300 _axiv, 104 pages :
_billustrations (some color) ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Chemical & Natural Resources Engineering
502 _aThesis (Master of Engineering (Chemical)) -- Universiti Malaysia Pahang – 2018
504 _aIncludes bibliographical references
520 3 _aLabisia pumila (locally known as Kacip fatimah) extract contains phenolic compounds such as gallic acid that have multiple biological effects. Ultrasound-assisted extraction (UAE) is widely reported for the extraction of medicinal plants and herbs due to its economical and green technology. Therefore, this study applied UAE with unified enzymatic extraction (EnE) to enhance the extraction yield of gallic acid from Labisia pumila. The influence of five parameters on the extraction of gallic acid from Labisia pumila were investigated: extraction time (1–8 h), sample-to-solvent ratio (1:6, 1:8, 1:10, and 1:12 wt/wt), temperature (40, 50, 60, and 80 °C), sonication (10%, 20%, and 40% duty cycle), and cellulase concentration (0.025, 0.05, 0.1, 0.2, and 0.3 g/L). The power intensity of 8.66 W/cm2 was implemented using an ultrasonic processor Q700 (700 watts, 20 kHz). The results showed that the gallic acid yield was the highest at 50 °C with 1:10 sample-to-solvent ratio for aqueous extraction (AE) with 1.0251 ± 0.0569 mg/g of gallic acid extracted. Whereas, both ultrasound-assisted extraction (UAE) and enzymatic extraction (EnE) techniques gave the highest yield at 40% duty cycle and 0.05 g/L cellulase concentration with 1.8425 ± 0.1191 mg/g and 1.28565 ± 0.1760 mg/g gallic acid extracted, respectively. The result of the ultrasound-assisted enzymatic extraction (UEnE) indicated an increased trend when the extraction time was increased from 1 to 7 h. However, beyond 7 h the yield declined indicating that the degradation of gallic acid may have initiated. The highest gallic acid amount obtained from UAEnE was 2.9287 ± 0.4060 mg/g after 7 h of extraction with 2.91-fold increment compared with the AE result. Thus, the integrated single unit ultrasonic-assisted enzyme extraction successfully improved the gallic acid extraction from Labisia pumila.
610 2 0 _aFaculty of Chemical & Natural Resources Engineering
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c7849
_d7855
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992