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005 20251117113405.0
008 180926t20182018my a f a m 001 0 eng d
020 _aTHE0000861(Local)
039 9 _a201905271629
_bnazirah
_y201809261549
_zfateeha
040 _aUMP
_beng
_cUMP
_erda
090 _aFKKSA .B39 2018 r Thesis
100 0 _aAhmad Bazli Bustary,
_eauthor.
245 1 0 _aBiological treatment of petrochemical effluent containing benzene, toluene and xylene (BTX) using pseudomonas putida ATCC49128 /
_cAhmad Bazli Bustary
264 1 _aKuantan, Pahang :
_bUMP,
_c2018
264 4 _c© 2018
300 _axiii, 129 pages :
_billustrations (some color) ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Chemical & Natural Resources Engineering
502 _aThesis (Master of Engineering (Chemical)) -- Universiti Malaysia Pahang – 2018
504 _aIncludes bibliographical references
520 3 _aBenzene, toluene, and xylene (BTX) are some examples of aromatic hydrocarbons that are found in petrochemical wastewater. Liquid and gaseous forms of the same are frequently present in municipal treatment plants. In this research, Pseudomonas putida ATCC 49128 was selected for use in the experiments owing to its ability to safely remove the aforementioned aromatic compounds without giving rise to the formation of other hazardous chemicals during the treatment of wastewater. The said experiments were aimed to study P. putida in terms of (1) its growth patterns under normal conditions and in concentrations of BTX, (2) its ability to remove different concentrations of BTX in a shake flask, as well as (3) its ability to remove BTX from actual petrochemical wastewater and simulated solutions. Repeated measurements of BTX concentrations at a petrochemical industrial wastewater effluent tank in Peninsular Malaysia revealed that the maximum values for benzene, toluene, and xylene were 55.2 ppm, 26.9 ppm, and 32.8 ppm respectively. Subsequently, the results of the treatment study showed that the optimal conditions for the growth of P. putida were an orbital shaker speed of 180 rpm, temperature of 37°C, pH 7, and P. putida-nutrient ratio of 1:9. Next, P. putida was made to remove different concentrations of simulated BTX for 48 hours; this procedure was repeated for actual petrochemical wastewater of pH 6, 7, and 8 respectively. This process was analysed using gas chromatography (GC-FID). Evidently, the bacteria were able to reduce the concentrations of BTX in actual petrochemical wastewater (of pH 7) by 85%, 77%, and 70% respectively; for the simulated BTX solution, the values were 87%, 80%, and 72% respectively. In the single simulated BTX solution, the reduction in the concentration of toluene (97%) exceeded those of benzene (93%) and xylene (62%). To conclude, the usage of P. putida ATCC49128 is an effective biological method to reduce the concentrations of BTX in petrochemical wastewater.
610 2 0 _aFaculty of Chemical & Natural Resources Engineering
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
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