000			03679ntm a2200373 i 4500
999			_c7856 _d7862
001			vtls000105309
003			KUKTEM
005			20251117113405.0
008			180926t20182018my a f a m 001 0 eng d
020			_aTHE0000145(Local)
039		9	_a201905131419 _bnazirah
040			_aUMP _beng _cUMP _erda
090			_aFIST .H35 2018 r Thesis
100	0		_aSiti Noor Hajar Zamrus, _eauthor.
245	1	0	_aSynthesis and characterization of curcuminoids by claisen-schmidt condensation and their cytotoxic effects on hela and K562 cancer cell lines / _cSiti Noor Hajar Zamrus
264		1	_aKuantan, Pahang : _bUMP, _c2018
264		4	_c© 2018
300			_axviii, 117 pages : _billustrations (some color) ; _c30 cm. + _e1 CD-ROM
336			_atext _2rdacontent
336			_atext _2rdacontent
337			_aunmediated _2rdamedia
337			_acomputer _2rdamedia
338			_avolume _2rdacarrier
338			_acomputer disc _2rdacarrier
347			_atext file _bPDF _2rda
500			_aFaculty of Industrial Sciences and Technology
502			_aThesis (Master of Science) -- Universiti Malaysia Pahang – 2018
504			_aIncludes bibliographical references
520	3		_aCancer is one of leading causes of death worldwide with the number of cases is expected to rise by 70% over the next two decades. These facts have lead researchers to continue studying how to treat cancer by find a new potential drug to replace the standard anti-cancer drugs such as doxorubicin which has side effects. Numerous natural compounds have been extensively investigated for their potential use in cancer prevention over decades. Curcumin from Curcuma longa has caught attention among researchers, is a highly promising natural compound that can potentially be used for chemoprevention of various cancers. Curcumin fulfils the characteristics for an ideal chemopreventive agent with its low toxicity, affordability and easy accessibility. But due to structurally instable, low solubility and poor bioavailability, the mono-ketone of curcuminoids were synthesized to improve its structure and poor bioavailability. About 20 curcuminoids were synthesized by using different substituted aldehydes and different ketones under Claisen-Schmidt condensation reaction method. The products were purified by using column chromatography, thin layer chromatography and crystallization processes. The chemical structures of the synthesized compounds were elucidated by using UV-VIS spectroscopy, Fourier transform infrared spectrometer (FTIR), Mass spectroscopy (MS) and Nuclear magnetic resonance spectrometer (NMR) spectroscopy. The compounds were screened for their cytotoxicity effects on HeLa human cervical carcinoma and K562 cancer cell lines by using MTT assay. Among these derivatives, 21 (IC50 = 12.50 ± 1.30), 22 (IC50 = 11.00 ± 2.80), 23 (IC50 = 9.00 ± 1.20), 26 (IC50 = 12.00 ± 1.60), 28 (IC50 = 11.00 ± 2.10), 29 (IC50 = 15.00 ± 1.60), 30 (IC50 = 15.00 ± 1.20), 31 (IC50 = 14.00 ± 1.40), 32 (IC50 = 11.00 ± 1.30), 33 (IC50 = 11.00 ± 1.20) and 34 (IC50 = 6.00 ± 1.20) showed high cytotoxic effects on HeLa human cervical carcinoma cell lines. Meanwhile, compounds 22 (IC50 = 6.50 ± 0.80), 23 (IC50 = 16.00 ± 1.30), 32 (IC50 = 15.00 ± 1.90) and 33 (IC50 = 12.50 ± 0.95) showed high cytotoxic effects against K562 cancer cell lines. Therefore, some compounds were found to show higher cytotoxicity effect against HeLa and K562 cancer cell lines in comparison to doxorubicin used as standard.
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aTheses
942			_2lcc _cTHESIS