000			03886ntm a2200313 i 4500
003			MY-KuUP
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007			ta
008			131122t20122012my a f m 000 0 eng d
020			_aTHE0008041(Local) _qhardback
040			_aUMP _beng _cUMP _erda
090			_aTP248.X95 R34 2012 rs Thesis
100	0		_aIslam, S. M. Rafiqul, _eauthor.
245		0	_aSynthesis of xylitol from meranti wood sawdust in batch process by locally produced xylose reductase / _cS. M. Rafiqul Islam
264		1	_aKuantan, Pahang : _bUMP, _c2012
264		4	_c©2012
300			_axxiii, 248 pages : _billustrations ; _c30 cm. + _e1 CD-ROM
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
500			_aFaculty of Chemical and Natural Resources Engineering
502			_aPhD (Doctor of Philosophy in Bioprocess Engineering) -- Universiti Malaysia Pahang - 2012
504			_aBibliography : p. 206-229
520	3		_aThe microbial production of xylitol has been studied extensively as an alternative to the chemical process, which on an industrial-scale is time-consuming mainly due to sterilization, inoculum development and product recovery problem. Apart from using pure xylose, no other substrate has been proposed for xylitol synthesis using xylose reductase (XR). The aim of this study was to synthesize xylitol, a specialty product, from Meranti wood sawdust hemicellulosic hydrolysate (MWSHH) by locally produced XR. MWS is a sawmill waste, which can be a promising source of xylose for xylitol production. The kinetic parameters of mathematical models were determined to predict xylose, glucose, furfural, and acetic acid concentration in the hydrolysate. Optimization of MWS hydrolysis was conducted with statistical design including onefactor-at-a-time (OFAT) method for maximum xylose recovery. The results of OFAT indicated the residence time, temperature, and acid concentration to be the major factors improving xylose recovery with a value of 60 min, 125 ºC, and 4%, respectively. These factors were further optimized by response surface methodology (RSM), and the optimum values obtained were 80 min, 124 ºC, and 3.26%, respectively. Under these conditions xylose yield and selectivity were attained at 90.6% and 4.05 g/g, respectively. XR was isolated from adapted C. tropicalis and characterized. The activity of NADPH-dependent XR measured was 11.16 U/mL. It was stable at pH 5.0–7.0 and temperature of 25–40 ºC for 24 h, and retained above 95% of its original activity after 4 months of storage at –80 ºC. The K values of XR for xylose and NADPH were 81.78 mM and 7.29 µM while the Vmaxm for xylose and NADPH were 178.57 and 12.5 µM/min, respectively. The high V max and low K values of XR for xylose reflecting a highly productive reaction among XR and xylose. Further, a sequential optimization based on OFAT approach and statistical program was followed to enhance xylitol production from xylose-rich MWSHH using XR. Firstly, the OFAT method coarsely evaluated the optimum levels of variables. Secondly, the significant variables for maximum xylitol production were screened out through fractional factorial design (FFD) to be reaction time, temperature and pH. Finally, the significant variables were fine-tuned by RSM and were found to be 12.25 h, 35 ºC and 6.5, respectively, giving the maximum xylitol yield and productivity of 86.57% and 1.33 g/L· h, respectively. Optimization of process conditions using sequential strategies resulted in 1.55-fold improvement in overall xylitol synthesis. This study developed a novel reaction medium to improve xylitol production to a considerable level using MWSHH.
650		0	_aXylose
856	4	0	_uhttps://umpir.ump.edu.my/id/eprint/37083/?template=default _zClick here to access online
942			_2lcc _cTHESIS
999			_c8643 _d8649