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008 190222t20182018my da f am 000 0 eng d
020 _aTHE0007810(Local)
039 9 _a201906131130
_bnadia
_y201902221616
_zsaini
040 _aUMP
_beng
_cUMP
_erda
090 _aFIST .T48 2018 r Thesis
100 0 _aTevan Ramanathan,
_eauthor.
245 1 0 _aGreen synthesis of silver nanoparticles from marine microalgae Isochrysis sp. and Amphora sp. through Ayurvedic method /
_cTevan Ramanathan
264 1 _aKuantan, Pahang :
_bUMP,
_c2018
264 4 _c© 2018
300 _axviii, 141 pages :
_billustrations (some color), chart ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Industrial Sciences and Technology
502 _aThesis (Doctor of Philosophy) -- Universiti Malaysia Pahang – 2018
504 _aIncludes bibliographical references
520 3 _aThe majority of metal nanoparticles are chemically synthesised, this is an environmentally risky, costly process and the produced nanoparticles are potentially toxic for biomedical and pharmacological applications. Therefore, green synthesis of metal nanoparticles is an interesting topic to explore. The study aims to identify and characterize marine microalgae species from Pantai Teluk Cempedak, Kuantan coastal region; biosynthesize and characterize silver nanoparticles (AgNPs) using Isochrysis sp. and Amphora sp. and finally determine the anticancer potential of synthesized AgNPs against human non-small cell lung carcinoma (NCL-H1299). Ayurvedic method was employed in this study to synthesize AgNPs. Physio-chemical properties were characterized by various analytical techniques such as UV-Vis spectrophotometer analysis, field emission scanning electron microscope (FESEM), energy-dispersive Xray spectroscopy (EDX), X-ray powder diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR). Further, antibacterial, antioxidant and anticancer potential of synthesised silver nanoparticles were investigated. As a result, nine species of marine microalgae were successfully identified and characterized, namely Coelastrum sp., Chlorococcum sp., Chlamydomonas moewusii, Scenedesmus sp., Nannochloropsis sp., Pavlova sp., Isochrysis sp., Amphiprora paludosa and Amphora sp. Out of nine species Isochrysis sp. and Amphora sp., were selected as potential species to synthesize AgNPs. The synthesized AgNPs were monodispersed and spherical in shape. The size was 98 to 193 nm for Isochrysis sp. and 42.2 to 50.8 nm for Amphora sp. Since microalgae is rich with secondary metabolites, reduction and stabilization of Ag+ ions to Ag° ions took place in room temperature within 3 hours. On the other hand, synthesized AgNPs were successfully inhibited the growth of Gram-negative bacteria Escherichia coli and Proteus vulgaris; Gram-positive bacteria Enterococcus faecalis and Bacillus subtilis. Furthermore, antioxidant activity of AgNPs was found to increase in a dose dependent manner. In addition, AgNPs have shown a reduction in cell viability and an increase in cytotoxicity on human non-small cell lung carcinoma (NCL-H1299). The cytotoxicity effects were observed to be significant from the concentration of 100 μg/ml AgNPs. Finally, the MRNA gene expression revealed that Isochrysis sp. and Amphora sp. Arishtas derived nanoparticles were able to induce the pro-apoptotic genes. Overall, these findings proved that the microalgae arishtas successfully synthesised AgNPs which acts as potent antibacterial, antioxidant and anticancer substances.
610 2 0 _aFaculty of Industrial Sciences and Technology
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c8795
_d8801
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992