000			02086nam a2200241 a 4500
001			vtls000045111
003			KUKTEM
005			20251114204354.0
008			100208t2009 my a f 000 0 eng d
020			_aTHE0002279(Local)
039		9	_a201905241213 _bshamsul _c201206191416 _dida _c201107132106 _dVLOAD _y201002081624 _zkam
040			_aUMP
090			_aQR84.5 .I58 2009 rs Bc.
100	0		_aIntan Noorleana Rosdi
245	1	0	_aGrowth optimization of N-fixer, isolated from biofertilizer / _cIntan Noorleana Bt Rosdi
246	3		_aGrowth optimization of n-fixer, isolated from _h[electronic resource]
260			_aKuantan : _bUMP, _c2009
300			_a35 p. : _bill. (some col.) ; _c30 cm. + _e1 computer disc
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009
520	3		_aThe objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standard curves needs to be constructed; growth, glucose and CFU curves. These three curves will be used in optimizing the growth condition of N-fixer. Set of experimental works were designed by DOE software using full factorial function. Based on the experiment results, it shows that the optimum pH and temperature for the growth of N-Fixer is at pH 6 and 30°C respectively. As for the requirement of carbon sources, 10 g/L was applicable to avoid the surplus glucose become toxic to microorganisms. As the conclusion, the concentration of glucose plays a major role in the fermentation process (18 hours) either to increase the number of N-fixer cell or to decrease it. Therefore, by maintaining it at 10g/L the number of cell can achieve the upper limit to where it can be produced which is about 1.6834E8 cell/mL .
650		0	_aBacterial growth
999			_aVIRTUA40 _c892 _d898
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*9992