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008			201012t20192019M a|||fram|| 000 0 eng d
020			_aTHE0008801(Local) _qhardback
040			_aUMP _beng _cUMP _erda
090			_aFKKSA .A43 2019 r Thesis
100	0		_aMohamad Al Aamin Razali, _eauthor.
245	1	0	_aSolid state fermentation of monascus purpureus in stirred drum bioreactor for production of red pigment from oil palm frond / _cMohamad Al Aamin Razali
264		1	_aKuantan, Pahang : _bUMP, _c2019
264		4	_c© 2019
300			_axi, 86 pages : _billustrations (some color) ; _c30 cm. + _e1 CD-ROM
336			_atext _2rdacontent
336			_atext _2rdacontent
337			_aunmediated _2rdamedia
337			_acomputer _2rdamedia
338			_avolume _2rdacarrier
338			_acomputer disc _2rdacarrier
347			_atext file _bPDF _2rda
500			_aFaculty of Chemical and Natural Resources Engineering
502			_aThesis (Master of Engineering (Bio-Process)) -- Universiti Malaysia Pahang – 2019
504			_aIncludes bibliographical references
520	3		_aVarious concern have been reported regarding the effect of existing synthetic colorant, hence, mass production of natural colorant seems to be a potential alternative in fulfilling the demand of colorant in industries. This research has emphasized on the process capability of stirred drum bioreactor on performing solid state fermentation (SSF) of Monascus purpureus FTC 5357 by using oil palm frond (OPF) for production of red pigment. Seven parameters, namely, initial moisture content (% v/w), inoculum size (spores/mL), peptone concentration (% w/w), initial pH, aeration rate (vvm), loading capacity (% v/v) and agitation programme (cycles/day) were evaluated for red pigment production, biomass, and glucose consumption. One Factor at A Time (OFAT) and two level factorial screening designs were used in the study. Three responses, which were red pigment production, biomass, and glucose consumption have been used as responses to identify the significant factors affecting the SSF process. In OFAT studies, the highest pigment production was recorded at aeration rate of 1 vvm (6.09 AU/g) and the lowest production was obtained at 45% of initial moisture content (0.1 AU/g). In two level factorial design experimental studies, the highest pigment production was 71.86 AU/g by incorporating 75% v/w of initial moisture content, 108 spores/mL of inoculum size, initial pH of 4, aeration rate of 1.21 vvm, loading capacity of 35 % v/v and agitation programme of 6 cycles/day. The most significant factor contributing to the red pigment production was initial moisture content, with F-value and p-value of 46.54 and 0.0002, respectively, followed by inoculum size, with F-value of 14.12 and p-value of 0.0071, respectively. Similar observation also occurred for biomass, shows that highest contribution from initial moisture content with F-value of 55.07 and p-value of 0.0051, followed by peptone with F-value of 20.47 and p-value of 0.0202, respectively. In conclusion, this work demonstrated the potential of using stirred drum bioreactor and OPF substrate in producing higher yield of red pigment production (71.86 AU/g) compared to shake flask fermentation (2.3 AU/g).
610	2	0	_aFaculty of Chemical and Natural Resources Engineering _xDissertations
650		0	_aUniversities and colleges _xDisertations
650		0	_aTheses
942			_2lcc _cTHESIS