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005			20251125105823.0
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008			201123t20122012m a|||fram|| 000 0 eng d
020			_aTHE0008994(Local) _qhardback
040			_aUMP _beng _cUMP _erda
090			_aFIST .C67 2012 r Bc.
100	1		_aCornelia, Chin Siew Ling, _eauthor.
245	1	0	_aIsolation and purification of pinellic acid from pinellia ternata / _cCornelia Chin Siew Ling
264		1	_aKuantan, Pahang : _bUMP, _c2012
264		4	_c© 2012
300			_axiv, 47 pages : _billustrations (some color) ; _c30 cm. + _e1 CD-ROM
336			_atext _2rdacontent
336			_atext _2rdacontent
337			_aunmediated _2rdamedia
337			_acomputer _2rdamedia
338			_avolume _2rdacarrier
338			_acomputer disc _2rdacarrier
347			_atext file _bPDF _2rda
500			_aFaculty of Industrial Sciences and Technology
502			_aProject Paper (Bachelor of Applied Science (Honors) in Industrial Chemistry) -- Universiti Malaysia Pahang – 2012
504			_aIncludes bibliographical references
520	3		_aPinellia ternata is a perennial herb belonging to the Araceae family. Pinellia ternata is a plant native to Japan, but also grows as a weed in certain areas of North America. It is commonly used as one of the main components in many decoctions in traditional Chinese medicine that has been used widely for thousands of years in China to prevent vomiting and for analgesic and sedative effects. Pinellia ternata is an active herbal component of the traditional Japanese herbal (Kampo) medicine, Sho-seiryu-to (SST) (Chinese name: Xiao-Qing-Long-Tang), and it has been reported to show oral adjuvant activity for nasally administered influenza hemagglutinin (HA) vaccine. The active compound that displayed adjuvant properties was identified as 9S, 12S, 13S-trihydroxy-10E-octadecenoic acid commonly known as Pinellic acid. This research was conducted with a goal to isolate and purify Pinellic Acid (9S, 12S, 13S-trihydroxy-10E-octadecenoic acid) from Pinellia ternata plant. Pinellia ternata was extracted with two types of solvent; water and methanol. The extracts were purified with Hydrophobic Interaction Chromatography with methanol as the polar eluent and Column Chromatography with the solvent system hexane-ethyl acetate. The pure Pinellic acid obtained was determined by Reversed phase High Performance Liquid Chromatography (RP-HPLC) using 70% methanol containing 0.01% acetic acid as the solvent system; and Infrared Spectroscopy. Highest % yield of Pinellic acid calculated was obtained from the sample extracted with methanol. High Performance Liquid Chromatography analysis for methanol-extract gave a clear single peak at 4.11 minutes at a flow rate of 1 ml/min. The compound obtained has displayed solvent selectivity towards methanol as opposed to water.
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and Colleges _xDissertations
650		0	_aTheses
942			_2lcc _cPSM