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008			220324s2021 my a|||frm||| 000 0 eng d
020			_aTHE0009184(Local) _qhardback
040			_aUMP _beng _cUMP _erda
090			_aFTKKP .F38 2021 r Thesis
100	1		_aNur Fathin Shamirah Daud, _eauthor.
245	1	0	_aOptimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357 / _cNur Fathin Shamirah Daud
264		1	_aKuantan, Pahang : _bUMP, _c2021
264		4	_c© 2021
300			_axiv, 118 pages : _billustrations (some color) ; _c30 cm. + _e1 CD-ROM
336			_atext _2rdacontent
336			_atext _2rdacontent
337			_aunmediated _2rdamedia
337			_acomputer _2rdamedia
338			_avolume _2rdacarrier
338			_acomputer disc _2rdacarrier
347			_atext file _bPDF _2rda
500			_aFaculty of Chemical and Process Engineering Technology
502			_aThesis (Master of Science) -- Universiti Malaysia Pahang – 2021
504			_aIncludes bibliographical references
520	3		_aMonascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimize pigments production (i.e. yellow, orange and red) from Monascus purpureus FTC 5357 using oil palm frond (OPF) in 5 L stirred-drum bioreactor. Prior to the SSF process, hydrothermal treatment (121 °C, 15 min) was done. A three-level Box–Behnken design (BBD) was used for the optimization, with threeparameters such as initial moisture content (IMC), aeration rate (AR) and peptone concentration (PC). The dried fermented OPF were extracted and analysed for pigments production (i.e. yellow, orange and red), biomass and glucose concentration. Next, the kinetic profile of the optimize condition and kinetic parameters were also been evaluated. Based on the ANOVA analysis results, IMC, AR and PC contributed significantly to the pigments production. The optimal fermentation conditions resulted IMC at 70 % w/w, AR at 1.30 vvm and PC at 4.40 % w/w. Under these conditions, the pigments production were obtained at 22.03, 20.11 and 18.59 Au/g.d for yellow, orange and red, respectively. Validation experiment was done to confirm the adequacy of the models. The fermentation profile at optimized condition indicated that Monascus purpureus FTC 5357 reached maximum pigments and biomass at day 9. Results obtained from this study showed the importance of IMC, AR and PC for pigments production and biomass. Meanwhile, the production kinetic parameters such as maximum specific growth rate, the growth yield, pigment yield, cell productivity, pigments productivity for yellow, orange and red were 0.707 day-1, 0.15 mg/mg, 0.193 Au/mg cell and 12.514 mg/(g.day), 2.447 Au/(g.d.day), 2.234 Au/(g.d.day) and 2.065 Au/(g.d.day), respectively. The pigments produced through SSF process using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future.
610	2	0	_aFaculty of Chemical and Process Engineering Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aTheses
942			_2lcc _cTHESIS