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008			220914t20222022my a|||fr|||| 000 0 eng d
020			_aTHE0009430 (Local) _qhardback
040			_aUMP _beng _cUMP _erda
090			_aKK .R34 2022 r Thesis
100	1		_aRafidah Mohamed Ali Hanapiah, _eauthor.
245	1	0	_aProduction of xylonic acid from xylose using recombinant E. Coli bl21 (DE3): _beffect of medium requirement / _cRafidah Mohamed Ali Hanapiah
264		1	_aPahang : _bUMP, _c2022
264		4	_c© 2022
300			_axiii, 72 pages: _bIllustration ; _c30 cm.+ _e1 CD ROM
336			_atext _2rdacontent
336			_atext _2rdacontent
337			_aunmediated _2rdamedia
337			_acomputer _2rdamedia
338			_avolume _2rdacarrier
338			_acomputer disc _2rdacarrier
347			_atext file _bPDF _2rda
500			_aCollege of Engineering
502			_aThesis (Master of Science) -- Universiti Malaysia Pahang – 2020
504			_aIncludes bibliographical reference
520	3		_aXylonic acid (XA) is a valuable organic acid that has various applications similar to other sugar acids such as gluconic acid (GA). Xylonic acid can be used in food, pharmaceutical and chemical industries as it has similar characteristics as sugar acids. Some of the applications include the function as complexing agent or chelator, in dispersal of concrete and as precursor for compounds such as co-polyamides, hydrogels and 1,2,4-butanetriol. In this study, recombinant Escherichia coli (E.coli) BL21 (DE3) was used to produce xylonic acid by manipulating a few parameters such as growth medium; Luria Bertani (LB), Super Optimal Broth (SOB),Terrific Broth (TB), M9 Minimal Medium (M9), and 2×Yeast-Tryptone (2×YT), nitrogen sources (yeast and tryptone concentration), inducer of isopropyl ß-D-1-thiogalactopyranoside (IPTG) concentration (0.01, 0.05, 0.1, 0.5 and 1.0 mM), induction time (2, 3, 4, 5 and 6 h), substrate concentration as well as comparison of substrate from difference sources. Fermentation samples were analysed using hydroxamate method and DNS method to determine XA and xylose concentration from fermentation process at 24 h, 200 rpm and 37 °C. The recombinant E.coli BL21 (DE3) was exploited as an alternative to the existing producers, with the ability to enhance the conversion of xylose to xylonic acid, fast growth, easy to handle and robust. From the study, the best culture conditions for XA production in recombinant E.coli BL21 (DE3) was achieved in Super Optimal Broth (SOB) medium, 20 g/L of tryptone, 5 g/L of yeast extract, 0.5 mM of IPTG with 2 h induction time and 10 g/L of D-xylose as substrate. Concentration of XA as high as 8.69 g/L from 10 g/L xylose was obtained when fermentation was governed under the best culture conditions when comparing with oil palm frond (OPF) hydrolysate. The result during the fermentation of OPF hydrolysate shows low concentration of XA. This is due to the limitation of xylose efficiency metabolism which affected the growth rate of recombinant E. coli BL21 (DE3). These results suggest a promising industrial-scale production of XA from recombinant E. coli BL21 (DE3).
610	2	0	_aCollege of Engineering
650		0	_aUniversities and colleges
650		0	_aThesis
942			_2lcc _cTHESIS