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008			230420t20232023my a|||fr|||| 000 0 eng d
020			_aTHE0009633 (Local) _qHardback
040			_aUMP _beng _cUMP _erda
090			_aFTKKP .N33 2023 r Thesis
100	1		_aNurul Nabila Huda Binti Baharudin, _eauthor.
245	1	0	_aImmobilization of escherichia coli producing β-cyclodextrin glucanotransferase on hollow fiber membrane for β-cyclodextrin production / _cNurul Nabila Huda Binti Baharudin
264		1	_aKuantan, Pahang : _bUMP, _c2023
264		4	_c©2023
300			_axiv, 105 pages : _billustrations (some color) ; _c30 cm. + _e1 CD-ROM
336			_2rdacontent _atext
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
337			_2rdamedia _acomputer
338			_2rdacarrier _avolume
338			_2rdacarrier _acomputer disc
347			_2rda _atext file _bPDF
500			_aFaculty of Chemical and Process Engineering Technology
502			_aThesis (Master of Science) -- Universiti Malaysia Pahang – 2023
504			_aIncludes bibliographical references
520	3		_a-cyclodextrin is a cyclic oligosaccharide that has been in high demand in various industries due to its numerous applications. It is produced from starch via the enzymatic conversion of β-cyclodextrin glucanotransferase (β-CGTase). While researchers have discovered a method to produce a high concentration of β-CGTase in the medium through excretion by recombinant Escherichia coli (E. coli), cell lysis remains an open problem as hydrostatic pressure builds up in the cytoplasm and periplasmic space of the E. coli. Cell immobilization is a promising solution to reduce cell lysis and enhance enzyme excretion. In the present study, the recombinant E. coli was immobilized using hollow fiber membrane. The production of -CD was optimized and the reusability of the immobilized cells were evaluated. The effects of substrate concentration, temperature, agitation rate, pH, and time on β-CD production, β-CGTase excretion, and cell lysis by the immobilized cells were determined by using the one factor at a time (OFAT) method. The results revealed that the immobilized cells could produce 11-14-fold more -CD compared to the free cells. The immobilized cells also exhibited a 17-19-fold increase in β-CGTase excretion with a 64-92% reduction of cell lysis compared to free cells. Then, the operating parameters was screened to identify the significant parameters using Full Factorial Design (FFD). It showed that the substrate concentration, temperature and agitation rate were the significant operating parameters and were used for optimization process using Central Composite Design (CCD) under Response Surface Methodology (RSM). Under the optimized conditions (3.9% of starch concentration, 44°C of reaction temperature, and 170 rpm agitation rate), the -CD production and β-CGTase excretion was 8-fold and 7-fold, respectively higher than before the optimization process. Moreover, the immobilized cells showed 3-fold and 2.5-fold of -CD production and β-CGTase excretion, respectively with 54% reduction of cell lysis in comparison with the free cells. The immobilized cells successfully retained up to 62% of the initial activity and can be reused for 5 cycles for the production of -CD. Therefore, the immobilization of E. coli on hollow fiber membrane was suitable for enhancing -CD production with high excretion of β-CGTase and high cell stability.
610	2	0	_aFaculty of Chemical and Process Engineering Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aTheses _xDissertations
942			_2lcc _cTHESIS