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Effect of murashige and skoog medium strength and plant growth regulators on the growth of momordica cochinchinesis (gac fruit) / Grace Ng Yun Ning

By:

Yun Ning, Grace Ng [author.]

Material type: Text

text

Media type:

unmediated

Carrier type:

volume

ISBN:

THE0010295 (Local)

Subject(s):

Online resources:

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Dissertation note: Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 Abstract: Momordica cochinchinensis, or Gac fruit, is a tropical superfruit known for its high nutritional and medicinal value but faces cultivation challenges such as low seed viability, poor germination, and environmental stress. Tissue culture offers a solution by enabling controlled propagation of elite plants. This study aimed to improve sterilization and optimize growth media for better in vitro propagation. Sodium hypochlorite concentrations for surface sterilization, Murashige and Skoog medium strengths, and cytokinin concentrations were evaluated for their role in affecting shoot growth and proliferation. Best surface sterilization was under 15% NaOCl for 10 minutes where 0% contamination rate was achieved, ensuring aseptic explant preparation. Among the tested MS medium strengths, full, half, and quarter, full-strength MS provided the best results, with the highest number of shoots (6.33 ± 0.33), shoot length (1.80 ± 0.06 cm), and leaf formation (4.67 ± 0.67) after five weeks. The study also tested various BAP concentrations (0, 1.0, 2.0, 3.0, and 4.0 mg/L), with 4.0 mg/L showing the highest shoot proliferation (16.67 ± 0.88), shoot length (4.50 ± 0.17 cm), and leaf number (12.00 ± 1.15), emphasizing the importance of hormonal optimization. The results highlight the significance of optimizing sterilization methods and media formulations to improve Gac fruit tissue culture efficiency, providing a foundation for sustainable cultivation and commercial production. Statistical analysis using One-Way ANOVA (p ≤ 0.05) showed that MS strength significantly affected all growth parameters, while BAP concentration significantly influenced shoot length but had no significant effect on shoot and leaf numbers

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Holdings
Item type	Current library	Call number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	FIST .Y86 2025 r Bc. (Browse shelf(Opens below))	Not for loan		T000003674

Faculty of Industrial Sciences and Technology

Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025

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Momordica cochinchinensis, or Gac fruit, is a tropical superfruit known for its high nutritional and medicinal value but faces cultivation challenges such as low seed viability, poor germination, and environmental stress. Tissue culture offers a solution by enabling controlled propagation of elite plants. This study aimed to improve sterilization and optimize growth media for better in vitro propagation. Sodium hypochlorite concentrations for surface sterilization, Murashige and Skoog medium strengths, and cytokinin concentrations were evaluated for their role in affecting shoot growth and proliferation. Best surface sterilization was under 15% NaOCl for 10 minutes where 0% contamination rate was achieved, ensuring aseptic explant preparation. Among the tested MS medium strengths, full, half, and quarter, full-strength MS provided the best results, with the highest number of shoots (6.33 ± 0.33), shoot length (1.80 ± 0.06 cm), and leaf formation (4.67 ± 0.67) after five weeks. The study also tested various BAP concentrations (0, 1.0, 2.0, 3.0, and 4.0 mg/L), with 4.0 mg/L showing the highest shoot proliferation (16.67 ± 0.88), shoot length (4.50 ± 0.17 cm), and leaf number (12.00 ± 1.15), emphasizing the importance of hormonal optimization. The results highlight the significance of optimizing sterilization methods and media formulations to improve Gac fruit tissue culture efficiency, providing a foundation for sustainable cultivation and commercial production. Statistical analysis using One-Way ANOVA (p ≤ 0.05) showed that MS strength significantly affected all growth parameters, while BAP concentration significantly influenced shoot length but had no significant effect on shoot and leaf numbers