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Optimization of fermentation for biopolymer production in shake-flask / Rosmerah Liaw

By:

Rosmerah Liaw

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2008Description: 36 p. : ill. (some col.) ; 30 cm. + 1 computer discISBN:

THE0004136(Local)

Other title:

Optimization of fermentation for biopolymer production in shake-flask [electronic resource]

Subject(s):

Dissertation note: Project paper (Bachelor of Chemical Engineering) --- Universiti Malaysia pahang - 2008 Abstract: Polyhydroxyalkanoates (PHA) are linear polyester produced in nature by bacterial fermentation of sugar or lipids that was first discovered by Lemoigne in 1925. Poly-Β-Hydroxybutyrate (PHB) is the most well known member of the PHAs. PHB is natural, biodegradable polyester, which is accumulated in the form of intracellular granules by a large variety of bacteria. These granules act as energy reserve materials when nutrients such as nitrogen and phosphorus source are available in limiting concentration in the presence of excess carbon source. This research studies the effect of variables in optimizing the production of PHB from bacteria Cupria vidus necator. In this study, the variables that being studied is temperature (28°C-35°C), glucose concentration (10g/L – 30g/L) and agitation speed (150rpm-300rpm). The purpose of this study is to find the value of media variable that will optimize the production of PHB in the fermentation process of biopolymer production. From the experiment and calculation, the maximum yield of biomass and PHB is achieve at the temperature of 34C, glucose concentration of 15.63g/L and at agitation of 222.83rpm. The maximum predicted yield for biomass (cell dry mass) is 3.07855g/L and the maximum predicted yield for PHB is 0.564093g/Lo. -Author

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Holdings ( 2 )
Title notes ( 2 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	TP156.F4 R67 2008 rs Thesis (Browse shelf(Opens below))	1	Not for loan		0000031409
Final Year Report	UMPLIB GAMBANG	CD 2768 \| TP156.F4 R67 2008 rs Thesis (Browse shelf(Opens below))	1	Not for loan		0000031410

Project paper (Bachelor of Chemical Engineering) --- Universiti Malaysia pahang - 2008

Polyhydroxyalkanoates (PHA) are linear polyester produced in nature by bacterial fermentation of sugar or lipids that was first discovered by Lemoigne in 1925. Poly-Β-Hydroxybutyrate (PHB) is the most well known member of the PHAs. PHB is natural, biodegradable polyester, which is accumulated in the form of intracellular granules by a large variety of bacteria. These granules act as energy reserve materials when nutrients such as nitrogen and phosphorus source are available in limiting concentration in the presence of excess carbon source. This research studies the effect of variables in optimizing the production of PHB from bacteria Cupria vidus necator. In this study, the variables that being studied is temperature (28°C-35°C), glucose concentration (10g/L – 30g/L) and agitation speed (150rpm-300rpm). The purpose of this study is to find the value of media variable that will optimize the production of PHB in the fermentation process of biopolymer production. From the experiment and calculation, the maximum yield of biomass and PHB is achieve at the temperature of 34C, glucose concentration of 15.63g/L and at agitation of 222.83rpm. The maximum predicted yield for biomass (cell dry mass) is 3.07855g/L and the maximum predicted yield for PHB is 0.564093g/Lo. -Author